BD, 559827, BD Pharmingen™ Purified NA/LE Mouse Anti-Mouse Qa-1[b]

Reactivity: Mouse (QC Testing)
Isotype: Mouse B6-Tla(a), also known as B6-Tlaª or QA-1ª IgG1, κ
Immunogen: Qa-1[b] aa. 161-179 Peptide
Application: Flow cytometry (Routinely Tested), Blocking, Immunoprecipitation, Western blot (Reported)
Concentration: 1.0 mg/ml
RRID: AB_397344
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Flow cytometry: Since this antigen is expressed at low density on the cell surface, it may be desirable to amplify staining by using a biotinylated second-step antibody (such as anti-mouse IgG1 mAb A85-1, Cat. No. 553441) followed by a "bright" third-step reagent such as PE Streptavidin (Cat. No. 554061).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.