BD, 560418, BD™ Cytometric Bead Array (CBA) Human Soluble CD14 Flex Set

Assay Range: 40-10,000 pg/mL
Reactivity: Human (QC Testing)
Regulatory Status: RUO
RRID: AB_2869341
Description: Description The BD™ CBA Human Soluble CD14 Flex Set is a bead-based immunoassay capable of measuring human soluble CD14 in cell culture supernatant samples. Human reactivity was determined by testing samples with the BD CBA Human Soluble CD14 Flex Set. The biology and function of CD14 has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264 or 558265). This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 ml polypropylene tube for reconstitution. When reconstituted in 4.0 ml Assay Diluent, the standard has a protein concentration of 10,000 pg/ml. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect PE Detection Reagent from prolonged exposure to light.
Recommended Assay Procedures: Recommended Assay Procedures The BD CBA Human Soluble CD14 Flex Set must be used in conjunction with a BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264, 100 tests, or 558265, 500 tests), a flow cytometer, and the FCAP Array™ Software (Cat. No. 641488). Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Human Soluble CD14 Flex Set will be 10,000 pg/ml. An example standard curve is shown in Figure 1. Due to high levels of sCD14 in serum and plasma samples, a starting dilution of 1:200 is recommended. The BD CBA Human Soluble CD14 Flex Set should not be used in the same assay well with any non-BD CBA Human Soluble Protein Flex Set or CD Marker Flex Set reagents (such as BD CBA Mouse Soluble Protein or Cell Signaling Flex Sets) nor with any BD CBA Human Soluble Receptor Protein Flex Set reagents . For technical protocols and additional resources, please visit "Bead-Based Immunoassays" at our website: http://www.bdbiosciences.com/us/s/resources. Performance Limit of Detection: The theoretical limit of detection is 9.2 pg/ml and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg/ml, n =30) + 2 standard deviations.