Qiagen, 333986, QIAseq Multimodal HT SW 96 UDI

QIAseq Multimodal HT SW 96 UDI contains DNA and RNA indexing for 96 samples using the separated workflow for targeted enrichment

Features

- Complete system to go from total nucleic acids to a sequencing-ready library using a single-day workflow
- Error correction with Unique Molecular Indices (UMIs) to enhance NGS panel sensitivity
- QIAseq enrichment technology overcomes the limitations of traditional amplicon approaches on amplicon size and primer multiplexing
- Unique Dual Indices (UDIs) to reduce sample index hopping
- Sample to Insight solution for consolidated targeted DNA and RNA sequencing

Product Details

QIAseq Multimodal Panels have been developed for consolidated targeted DNA and RNA enrichment and analyses. Unlike other available approaches, QIAseq Multimodal Panels do not require 2 separate workflows for DNA and RNA analysis – saving time and conserving samples that are of limited availability.

Custom panels can be designed to target exonic regions of genes, hotspots or SNPs, intronic and promoter regions, known gene fusions based on characterized breakpoints, or to discover novel fusions.

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Principle

- Large amounts of sample material required for generating sufficient amounts of input DNA and RNA for multiple workflows
- Added complexity of deriving integrated insights from results of different technical approaches, each with its own innate bias
- Inefficient use of resources
- Long turnaround time

Recent advances in NGS chemistries, platforms, and bioinformatics pipelines are enabling users to efficiently interrogate biological samples for changes in DNA and RNA. Other available approaches, however, require the use of 2 separate workflows to prepare libraries from separate DNA and RNA isolates. Limitations of such approaches include:

To overcome the limitations associated with current approaches, QIAseq Multimodal Panels start with total nucleic acids (or DNA + RNA) and prepares UDI-containing, Illumina-compatible targeted DNA and RNA libraries using a single-day, consolidated workflow. In addition, QIAseq Multimodal Panels have been designed for use with low-yield and poor-quality biological samples.

Procedure

Workflow of the QIAseq Multimodal Panels

The QIAseq Multimodal Panel workflow can be used to prepare sequencing-ready libraries in a single day. The library insert size is approximately 150 bp, making the QIAseq Multimodal Panels highly compatible with low-quality samples, such as FFPE samples.

Robust detection of DNA and RNA biomarkers

QIAseq Multimodal Panels can be used to reliably detect DNA and RNA biomarkers using a consolidated workflow from total nucleic acids. The ability of QIAseq Multimodal Panels to simultaneously detect DNA and RNA biomarkers has been benchmarked against two separate workflows (namely, QIAseq Targeted DNA Pro and QIAseq Targeted Fusion XP).

Applications

- Single nucleotide variants (SNVs)
- Insertions and Deletions (InDels)
- Copy number variants (CNVs)

- Fusions
- Exon skipping events
- Gene expression levels

QIAseq Multimodal Panels can be used to interrogate different types of biomarkers using a consolidated workflow from total nucleic acids, which can be isolated using dedicated sample isolation protocols that have been developed specifically for the QIAseq Multimodal Panels.

From DNA:

From RNA: