Qiagen, 339332, miRCURY LNA Custom PCR Panel Additional Plates (4)

Additional miRCURY LNA Custom PCR Panel plates; set of 4 plates; only available in addition to the base plates ordered through the core product (cat. no. 339330)

Features

- Combine predesigned verified and custom-designed LNA PCR Assays in single-use, ready-to-use plates
- Customize for your favored plate type, layout, PCR instrument and batch size
- Great for sensitive, specific and reproducible miRNA profiling of a medium to large number of samples
- Use our new easy-to-use online design tool

Product Details

miRCURY LNA miRNA Custom Panels are ready-to-use 96- and 384-well plates containing individual miRCURY LNA miRNA PCR Assays according to your particular requirements. Both forward and reverse PCR amplification primers are miRNA-specific and are optimized with LNA technology , enabling extremely sensitive and specific miRNA quantification with the miRCURY LNA miRNA PCR System . Each well contains sufficient primer set for one 10 µl reaction.

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Performance

Top performance in specificity

The miRCURY LNA miRNA PCR System is the only miRNA quantification platform with absolute specificity (see the miRQC study published in Nature Methods ) and out-performed another probe-based miRNA qPCR platform in specificity tests (see figure Specificity test alongside competitor probe-based miRNA qPCR system ). This eliminates false positives and ensures only robust and reliable miRNA signals. The easy-to-follow, 3-hour protocol saves you both time and effort in the laboratory. Simply dilute the synthesized cDNA, combine it with the miRCURY LNA SYBR Green Master Mix and aliquot into the PCR plate, which is then ready to run in your real-time PCR instrument. The number of pipetting steps is reduced to a minimum, reducing technical variation. As a result, you can achieve extremely high reproducibility from day-to-day and even site-to-site (see figures Excellent day-to-day reproducibility and Excellent site-to-site correlation ). High reproducibility is even possible when profiling difficult samples, such as RNA purified from serum (see figure Excellent reproducibility between RT reactions on total RNA from serum ).

Principle

- 96-well, ready-to-use: design your panels from one of three available layouts. Choose from our vast selection of experimentally validated LNA miRNA PCR primer sets and reference gene primer sets for human, mouse and rat miRNA quantification.
- 384-well, ready-to-use: design your panels from one of four available layouts. Choose from our vast selection of experimentally validated LNA miRNA PCR primer sets and reference gene primer sets for human, mouse and rat miRNA quantification.

Coverage

Add custom assays

Applications

- Pathway- and disease-focused miRNA profiling
- Biomarker discovery