Qiagen, RP50, PCR Anti-Inhibitor (100)

100 μL of PCR Anti-Inhibitor, liquid Storage temperature: -20°C

Features

- Possesses a mixture of alkaline
- Eliminates inhibitors found in a DNA template isolation

Product Details

PCR Anti-inhibitor is a carefully composed mixture of alkaline proteins which counteracts substances inhibiting the PCR reaction. Adding PCR Anti-inhibitor to a reaction mixture is an ideal way of eliminating inhibitors originating in a DNA template isolation.

Performance

Assay: Specification
Functionality: Passed
DNase contamination: None detected

Principle

Quality Control

DNase activity was evaluated by incubating 1 μg of DNA with 1 μL of the enzyme for 4 hours at 37°C. Results are visualized on an ethidium bromide-stained agarose gel. The product was extensively tested in PCR reaction.

Usage

Add the PCR Anti-inhibitor to the PCR mixture in a 1:50 volume ratio, e.g., add 1 μL of the PCR Anti- inhibitor to 50 μL PCR reaction.

Applications

- PCR with difficult DNA templates isolated from samples such as urine, saliva, sputum, blood, cell swabs, cerebrospinal fluid, biopsies, etc.

This is used for application such as: