Waters, 186009839, XSelect Premier Peptide HSS T3 Column, 100Å, 2.5 µm, 2.1 x 50 mm

The XSelect Premier Peptide HSS T3 Column, 100Å, 2.5 µm, 2.1 x 50 mm Columns are designed for separations where silica-based selectivities are desired or when increased retention of hydrophilic peptides are required. Each batch of HSS T3, 100Å material is QC tested with a gradient separation of a tryptic digest of cytochrome c (p/n: 186006371) and must pass stringent performance criteria before acceptance for use in these columns. 2.1mm ID columns are recommended for use with low dispersion (UPLC or UHPLC) systems, especially for LC-MS methods. This column is part of the MaxPeak Premier class of columns, which deliver the chromatographic performance expected from our particle technologies while increasing reproducibility, peak shape, and recovery for metal-sensitive analytes. Suggested Use: Peptide Separations, Peptide Analysis

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Silica
pH Range Min: 2 pH
pH Range Max: 8 pH
Temperature Limits: 45 C
Maximum Pressure: 18000 psi (1240 Bar)
Endcapped: Yes
Bonding Technology: T3
Silanol Activity: Medium
Particle Shape: Spherical
Particle Size: 2.5 µm
Endfitting Type: Parker-style
Pore Size: 100 Å
QC Tested: Peptide
Format: Premier Column
Surface Area: 230
System: UHPLC, UPLC
Particle Technology: HSS
Technique: LC
USP Classification: L1
Inner Diameter: 2.1 mm
Length: 50 mm
Carbon Load: 11 %
eCord: Yes
UNSPSC: 41115709
Application: Peptide
Brand: XSelect
Product Type: Columns
Units per Package: 1 pk

Additional Information
XSelect Premier Peptide HSS T3 Column, 2.5 µm, 2.1 x 50 mm When performing protein separations or proteomics-related assays in general, there’s a series of considerations that a scientist must acknowledge and reply to with the proper equipment. That’s where the XSelect Premier Peptide HSS T3 Column comes into play. This silica column offers the silica-based selectivity desired by scientists working in related assays, and it’s equipped with MaxPeak High-Performance Surface technology to prevent unwanted analyte-surface interactions. Add all these incredible features up and you get a terrific chromatography consumable that you’ll want at your command in the lab. Tell your lab manager to add the XSelect Premier Peptide HSS T3 Column to your procurement list, or get your own XSelect Premier Column now to be better prepared for the science of tomorrow. Hydrophilic peptide retention is a huge priority for scientists working in proteomics, which is why the XSelect Premier Peptide HSS T3 Column was developed with that main objective as the top priority. The stable High-Strength Silica (HSS) particle enables this column to maintain mechanical stability under varying backpressures, temperatures and mobile phases, but the excellent low pH stability offered by this UHPLC column makes it a top choice among experts in the field. What’s more, the MaxPeak technology running through this analytical column helps to prevent any losses in retention due to analyte-surface interactions. Need to use a 100% aqueous mobile phase? You’re in luck, as the XSelect Premier Peptide HSS T3 Column is 100% water wettable, which also enables it to provide excellent reverse-phase retention of polar analytes. And because maximizing selectivity while minimizing variability is of the utmost importance to this XSelect Premier Column, you can perform method development with this silica column and seamlessly transfer methods afterwards. Just compare the XSelect Premier Peptide HSS T3 Column to other peptide-related consumables and you’re sure to see why people choose XSelect Premier time and again. Even if you’re not performing proteomics or peptide-related assays, there’s an XSelect Premier Column uniquely suited for your needs. With two innovative base particles to choose from and a long list of column chemistries offered, choosing an XSelect Premier Column should be an easy decision to make. Plus, every single XSelect Premier Column comes equipped with the MaxPeak High-Performance Technology mentioned earlier. This way you can be even more confident in your important assays. Shopping at Waters is a scientist’s dream, or that’s how we’d like to think the experience is like. From quality control reference materials for system benchmarking to lab informatics needed to compile data, we simply have it all. Shop for lab equipment now to learn more.

FAQ
What’s the XSelect Premier Peptide HSS T3 Column’s pH Range? The XSelect Premier Peptide HSS T3 Column offers a pH range of 2 to 8 pH.