BD, 551964, BD Pharmingen™ Purified Mouse Anti-Human Toll-Like Receptor 4

Alternative Name: TLR4; CD284; Toll-like Receptor 4; ARMD10
Reactivity: Human (QC Testing)
Isotype: Mouse BALB/c IgG2a, κ
Immunogen: Human TLR4-transfected Ba/F3 cell line
Application: Flow cytometry (Routinely Tested), Blocking (Reported)
Concentration: 0.5 mg/ml
RRID: AB_394304
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures A multistep step staining procedure is recommended to amplify immunofluorescent signals for the flow cytometric analysis of human TLR4 expression: Step 1: Incubate 10^6 cells with 0.25 - 1.0 µg/test of purified HTA125 antibody at 4°C for at least 15 - 20 minutes. Wash cells two times with staining medium containing sodium azide (e.g., BD Pharmingen™ Stain Buffer, Cat. No. 554656). Step 2: Incubate the cells with biotinylated anti-mouse Ig  (Cat. No. 553999) or biotinylated anti-mouse IgG2a, (Cat. No. 553388) at 4°C for 20 minutes. Wash cells two times. Step 3: Incubate the cells with  ≤0.06 µg of streptavidin-phycoerythrin (Cat. No. 554061) at 4°C for 20 minutes. Wash two times. Resuspend cells in stain buffer and analyze stained cells by flow cytometry using appropriate specificity and compensation controls.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. An isotype control should be used at the same concentration as the antibody of interest. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.