BD, 553577, BD Pharmingen™ Purified Mouse Anti-Mouse H-2D[d]

Reactivity: Mouse (QC Testing)
Isotype: Mouse C3H, also known as C3H/He, C3H/Bi IgG2a, κ
Immunogen: (C57BL/6 x DBA/2)F1 mouse splenocytes
Application: Flow cytometry (Routinely Tested), Blocking, Cytotoxicity, Immunoprecipitation (Reported)
Concentration: 0.5 mg/ml
RRID: AB_394935
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Other reported applications include complement-dependent cytotoxicity, immunoprecipitation, and blocking of EL4 cell adhesion to H-2D[d]. For immunohistochemical staining (IHC) of frozen sections expressing MHC class I antigen of the d haplotype, we recommend the use of biotinylated anti-mouse H-2K[d] mAb SF1-1.1, Cat. No. 553564. mAb SF1-1.1 is not recommended for IHC of formalin-fixed paraffin-embedded sections.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.