Waters, 176003688, ProteinWorks eXpress Direct Digest Kit

The ProteinWorks eXpress Direct Digest Kit simplifies and accelerates the direct digestion of whole, non-immunopurified plasma & serum samples for LC-MS protein quantification via the surrogate peptide approach. The kit includes pre-measured, lot-traceable reagents, a flexible 96-tube sample collection module, and optimized protocols, offering scientists a standardized, proven route to fast, accurate and precise protein quantification for protein bioanalysis. The ProteinWorks eXpress Direct Digest Kit contains: (96) Denaturant* Cluster Tubes, Cap Mat & Rack for high protein content samples (1.0 - 5.25 mg total protein per sample) (12) 8-Strip Caps for Cluster tubes (1) Digestion Buffer (1) Trypsin Dissolving Agent (1) Digestion Inactivation Agent (3) Reduction Agent vials. Each vial is enough for 32 samples (3) Alkylation Agent vials. Each vial is enough for 32 samples (1) Trypsin for high protein content samples (1.0 - 5.25 mg total protein per sample) *The denaturant is RapiGest™ SF, an enzyme friendly detergent used to denature proteins prior to digestion. For more information on RapiGest SF, see 720000468en.

Specifications
Number of Analysis: 96
Type: Sample Preparation Workflow
Packages per kit: 2
Separation Mode: RPLC
Detection Method: MS/MS
Storage Condition: Refer to the storage recommendations of each sub-package
System Type: LC-MS
Shelf Life: Refer to the storage recommendations of each sub-package
UNSPSC: 41116104
Application: Protein, Peptide
Brand: ProteinWorks
Product Type: Application Kits
Units per Package: 1 pk

Additional Information
ProteinWorks eXpress Direct Digest Kit The ProteinWorks eXpress Direct Digest Kit is a kit-based method that achieves single-digit intra-kit and inter-kit repeatability across a variety of monoclonal antibodies. With a validated, standardized, kit-based technique that is easily deployable from analyst to analyst, lab to lab, ProteinWorks kits help to ensure laboratory and data consistency. The ProteinWorks eXpress Direct Digest Kit makes it easier and faster to digest entire, non-immunopurified plasma and serum samples for LC/MS protein quantification using the surrogate peptide technique. The kit comes with pre-measured, lot-traceable chemicals, a flexible 96-tube sample collection module, and optimized protocols, giving scientists a standardized, tried-and-true method for determining protein concentrations quickly, accurately, and precisely. By combining the ProteinWorks Sample Preparation Kits with strong and reliable LC-MS methodologies and instrumentation, Discovery and Early Development laboratories can achieve consistent, reproducible, accurate protein quantification by using surrogate peptide methodology. In order to ensure that you receive authentic Waters equipment for your lab, we highly recommend that you browse through our website or catalog from where you can shop for lab equipment to fulfill your laboratory-specific needs and get the best market prices for our equipment. The website also enables you to browse through the entire collection of equipment available from Waters and ensures that the equipment you receive is authentic. You may also be interested in ACQUITY UPLC HSS T3 Column, 100Å, 1.8 µm, 2.1 mm X 150 mm, 1/pk; The universal, silica-based bonded phase used for the ACQUITY HSS T3 sorbents is compatible with 100% aqueous mobile phase and should be your first choice when developing separations for polar and non-polar compounds. The ACQUITY HSS T3 UPLC columns exhibit ultra-low MS bleed and are fully scalable and transferable to HPLC separations.

FAQ
Why Is It That My LC System Keeps Shutting Down? HPLC pumps and systems are equipped with safety measures to prevent overpressure and safeguard the integrity of columns and equipment. In the event of excessively low settings or excessive backpressure, the pump can automatically shut down to protect the entire LC system. Additional factors that may trigger such protective measures include the requirement for line cleanup or the elimination of residual or crystallized materials that could obstruct or impede the system's functionality. Ensuring proper maintenance and addressing potential obstructions are vital for uninterrupted and reliable HPLC operation. How Would You Describe Resolution In Terms Of Chromatography? Chromatographers can improve their sample separations by measuring resolution, which is a function of peak width and retention time, both of which are fundamental aspects of the chromatographic process.