Agilent, 5065-9911, ZORBAX 300SB-C18, 3.5 µm, 0.075 x 150 mm

ZORBAX 300SB-C18, 3.5 µm, 0.075 x 150 mm. A reversed-phase C18 HPLC column with fully porous ZORBAX particles. StableBond technology ensures stability at low pH.

Specifications:
Brand: ZORBAX 300SB-C18
Hardware: SS
Inner Diameter (ID): 0.075 mm
LC Platform: Stainless Steel
Length: 150 mm
Maximum Temperature: 90 °C
Part Number Application Domain: Biopharma
Part Number Application: Monoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 3.5 µm
Particle Type: Fully Porous
Phase: 300SB-C18
Pore Size: 300 Å
Pressure Rating: 400 bar
Separation Mode: Reversed Phase
Shipping Solvent: Acetonitrile/Water
Technique: LCLC & LC/MS
UNSPSC Code: 41115709
pH Range: 1-8

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The ZORBAX 300SB-C18 column offers advanced reversed-phase chromatography with performance optimized for peptide and protein separations. Featuring a 3.5 micron particle size and 300 angstrom pore diameter, this analytical column is engineered specifically for high-resolution analysis and purification of biomolecules with larger molecular weights. Its stable bonded silica surface ensures excellent peak shape and reproducibility, even under low pH and high aqueous mobile phase conditions.

Ideal for proteomics and biopharmaceutical research, the column enables detailed characterization of peptides, oligonucleotides, and proteins such as monoclonal antibodies. The 0.075 mm internal diameter is particularly suited for capillary and nano-flow LC applications, providing high sensitivity and efficient sample usage. Typical usage scenarios include LC-MS workflows for complex biological samples and low-abundance biomarker discovery.

Compatible with Agilent LC systems such as the 1200 Infinity Series and 1260 Infinity II, as well as other nano-LC platforms, this column integrates seamlessly into existing workflows. Pairing with ZORBAX 300SB series columns of varied dimensions supports scale-up and method development. The robust column chemistry and precise specifications make it a trusted choice for demanding research and routine analytical applications.