Agilent, 5069-3639, Bio-Monolith Protein A, 4.95 x 5.2 mm

Bio-Monolith Protein A, 4.95 x 5.2 mm. A biomonolith native Protein A column used to purify mouse, most human IgG subtypes, and suitable for some IgMs. Store columns at 4-8 °C before and after use, extra delivery cost for cold shipment.

Specifications:
Brand: Bio-Monolith
Hardware: SS
Inner Diameter (ID): 5.2 mm
LC Platform: Stainless Steel
Length: 5 mm
Maximum Temperature: 40 °C
Part Number Application: Antibody Titer Determination
Part Number Application Domain: Biopharma
Particle Type: Monolith
Phase: Protein A
Pressure Rating: 150 bar
Separation Mode: Affinity
Shipping Solvent: 20mM Tris + 20% Ethanol
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-11

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The Bio-Monolith Protein A, 4.95 x 5.2 mm column from Agilent is a robust solution designed for rapid and selective purification as well as quantitation of immunoglobulins directly from complex biological samples. Utilizing monolithic technology, this column offers high flow rates and low backpressure, enabling efficient high-throughput protein A affinity chromatography. Researchers commonly employ this column for the capture and analysis of monoclonal and polyclonal antibodies, antibody fragments, and other Fc-containing proteins, facilitating antibody characterization, titer determination, and drug development workflows.

The unique column design ensures reproducible performance and reduced run times, making it ideal for bioprocess development, quality control, and academic research. It integrates seamlessly with Agilent HPLC and UHPLC systems, including the 1260 Infinity and 1290 Infinity series, as well as other compatible chromatographic platforms. In addition, it works in conjunction with Agilent’s range of sample preparation products and protein quantitation kits to streamline analytical workflows. The column’s high specificity for antibody Fc regions ensures minimal nonspecific binding and robust recovery, supporting accurate result generation in both research and industrial laboratory settings.