Agilent, 5190-2445, Agilent Bio WCX, NP5, 4.6 x 250 mm

Agilent Bio WCX, NP5, 4.6 x 250 mm. A weak cation exchange HPLC column with nonporous particles. Particle size is 5 μm. Nonporous particles are best for efficient mass transfer and high resolution separations.

Specifications:
Brand: Agilent Bio WCX
Column Material: Stainless Steel
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 250 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: Charge Variant AnalysisPeptidesProteins
Particle Size: 5 µm
Particle Type: Non-porous
Phase: WCX
Pressure Rating: 413 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-12

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The Agilent Bio WCX NP5 column, sized 4.6 x 250 mm, is engineered for high-resolution separation of proteins, peptides, and other biomolecules using weak cation exchange chromatography. With its non-porous, polymer-based particle design, this column delivers superior mass transfer and consistent performance, enabling rapid and efficient analysis with excellent reproducibility. Ideal for applications in biopharmaceutical development, it supports tasks such as charge variant analysis of monoclonal antibodies, characterization of recombinant proteins, and quality control in research and manufacturing environments. The robust construction ensures stability under a wide range of pH and salt concentrations, facilitating gradient or isocratic elution methods. Compatible with Agilent HPLC and UHPLC systems as well as instruments from other leading manufacturers, this column is well-suited for integration into existing laboratory workflows. It also pairs effectively with Agilent’s full range of biocolumns and analytical standards for comprehensive bioseparation strategies. The reliable performance, versatility in handling complex biological samples, and easy method scalability make it a preferred choice for researchers seeking precise and reproducible cation exchange separations in regulated or discovery laboratories.