Agilent, 5190-2459, Agilent Bio WCX, NP10, 2.1 x 250 mm, PEEK

Agilent Bio WCX, NP10, 2.1 x 250 mm, PEEK. A weak cation exchange HPLC column with nonporous particles. Particle size is 10 μm. PEEK hardware enables this column to couple with bio-compatible or bio-inert LC systems.

Specifications:
Brand: Agilent Bio WCX
Column Material: PEEK
Hardware: PEEK
Inner Diameter (ID): 2.1 mm
LC Platform: BioInert
Length: 250 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: Charge Variant AnalysisPeptidesProteins
Particle Size: 10 µm
Particle Type: Non-porous
Phase: WCX
Pressure Rating: 275 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-12

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Designed for robust and high-resolution ion exchange chromatography, this analytical column features a weak cation exchange (WCX) stationary phase in a non-porous NP10, 2.1 x 250 mm PEEK format. The column is ideal for the separation, purification, and characterization of proteins, peptides, and other biomolecules, enabling precise charge variant analysis and streamlined workflows in biopharmaceutical development and quality control laboratories. Its non-porous 10 micron particle size ensures efficient mass transfer and sharp peak shapes, supporting accurate quantitation and increased throughput.

The durable PEEK hardware provides outstanding chemical resistance, minimizing metal-ion interactions and ensuring compatibility with a wide range of buffers and pH conditions. This column is suitable for integration with Agilent HPLC and UHPLC systems, as well as other major chromatographic platforms. Typical use cases include charge heterogeneity analysis for monoclonal antibodies, protein isoform profiling, and quality assessment of therapeutic formulations.

For seamless method development and scalability, the column works in conjunction with Agilent’s complete range of bioseparation columns and compatible software solutions, supporting compliance with analytical and regulatory requirements. Its reproducible performance and robust design make it an essential tool for scientists conducting advanced biomolecule characterization and development.