Agilent, 5190-2463, Agilent Bio SAX, NP3, 4.6 x 50 mm

Agilent Bio SAX, NP3, 4.6 x 50 mm. A strong anion exchange HPLC column with nonporous particles. Particle size is 3 μm. Nonporous particles are best for efficient mass transfer and high-resolution separations.

Specifications:
Brand: Agilent Bio SAX
Column Material: Stainless Steel
Guard Column: No
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 50 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: OligosProteins
Particle Size: 3 µm
Particle Type: Non-porous
Phase: SAX
Pressure Rating: 551 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-12

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This strong anion exchange chromatography column is designed for high-resolution separation of biomolecules, including proteins, peptides, and nucleic acids, in complex biological samples. Featuring non-porous 3-micron particles and a 4.6 x 50 mm format, it delivers rapid, high-efficiency purification and characterization of charged analytes in demanding research and biopharmaceutical workflows. Its robust chemistry ensures excellent reproducibility, high binding capacity, and superior resistance to fouling, making it suitable for analytical method development, quality control testing, and process monitoring in regulated environments.

The column is optimized for compatibility with Agilent HPLC and UHPLC systems, including InfinityLab LC Series, and can be seamlessly integrated into multi-dimensional separations or coupled with mass spectrometry for in-depth structural analysis. Typical applications include charge variant analysis of monoclonal antibodies, oligonucleotide separation, and purification of recombinant proteins or enzyme isoforms. The device is also suitable for screening post-translational modifications or product-related impurities in pharmaceutical research. Compatible accessories and related offerings include a full range of BioHPLC guard columns, fittings, and mobile phases from Agilent, supporting robust and reproducible workflows. Its durable design ensures extended lifespan and reliable performance across a wide range of applications.