Agilent, 5190-2495, Agilent Bio WAX, NP10, 4.6 x 250 mm, PEEK

Agilent Bio WAX, NP10, 4.6 x 250 mm, PEEK. A weak anion exchange HPLC column with nonporous particles. Particle size is 10 μm. PEEK hardware enables this column to couple with bio-compatible or bio-inert LC systems.

Specifications:
Brand: Agilent Bio WAX
Column Material: PEEK
Hardware: PEEK
Inner Diameter (ID): 4.6 mm
LC Platform: BioInert
Length: 250 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: OligosProteins
Particle Size: 10 µm
Particle Type: Non-porous
Phase: WAX
Pressure Rating: 275 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-12

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The Agilent Bio WAX NP10, 4.6 x 250 mm, PEEK column is designed for high-performance anion exchange chromatography with proteins, peptides, and biomolecules. Utilizing non-porous 10 µm particles, this column delivers rapid, high-efficiency separations and excellent resolution, minimizing sample diffusion and enabling faster analysis times. The PEEK construction provides outstanding chemical resistance, making it suitable for high-salt and high-pH mobile phases commonly employed in bioseparations. Its robust design supports reproducible, reliable separations for demanding analytical and preparative workflows.

Applications include protein variant analysis, monoclonal antibody characterization, charge isoform profiling, and quality control in biopharmaceutical research and manufacturing. The column consistently achieves precise quantitation and identification, supporting both method development and routine analysis in regulatory or research environments. This column is compatible with Agilent 1100, 1200, and 1260 Infinity LC systems, as well as comparable HPLC or UHPLC instruments from other major manufacturers when using standard 4.6 mm ID column hardware. It can be integrated with detectors such as UV, fluorescence, or mass spectrometry for comprehensive biomolecule analysis. For workflow optimization, Agilent offers a full line of sample preparation products and bio-inert LC accessories tailored for protein and peptide separations.