Agilent, 5190-2508, Agilent Bio SEC-3, 150Å, 4.6 x 300 mm, 3 µm

Agilent Bio SEC-3, 150Å, 4.6 x 300 mm, 3 µm. A size exclusion column for aggregate analysis of peptides and small proteins. Resolving range 0.5-150 kDa.

Specifications:
Brand: Agilent Bio SEC-3
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 300 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 500 Da
Molecular Weight Upper Limit: 150000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisPeptidesProteins
Particle Size: 3 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 150 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The Agilent Bio SEC-3 column with 150 Angstrom pore size, dimensions of 4.6 x 300 mm, and 3 micron particle size is designed for high-resolution size exclusion chromatography of biomolecules, including peptides, proteins, and small biotherapeutics. Its narrowly defined pore structure allows precise separation based on molecular size, making it ideal for applications such as aggregate analysis, purity assessment, and molecular weight determination in both research and quality control laboratories. The column supports rapid and accurate profiling of complex biological samples, helping researchers achieve reproducible results even with small sample volumes. Optimized for use with Agilent InfinityLab and 1100, 1200, and 1290 series HPLC and UHPLC systems, it also integrates seamlessly into many standard chromatography workflows. The durable silica-based stationary phase ensures stability over multiple runs and compatibility with a broad range of aqueous and organic mobile phases. The column performs effectively in protein characterization, formulation development, biosimilar analysis, and in the evaluation of antibody fragments or ADCs. Agilent offers a complete set of reagents and SEC accessories designed for optimal performance with this column, further supporting streamlined workflow in biopharmaceutical and life sciences research environments.