Agilent, 5190-2524, Agilent Bio SEC-5, 150Å, 4.6 x 150 mm, 5 µm

Agilent Bio SEC-5, 150Å, 4.6 x 150 mm, 5 µm. A size exclusion column for aggregate analysis of peptides and small proteins. Resolving range 0.5-150 kDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 150 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 500 Da
Molecular Weight Upper Limit: 150000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisPeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 150 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The Agilent Bio SEC-5 column with 150 angstrom pore size, 4.6 x 150 mm dimensions, and 5 micron particle size is engineered for high-resolution size exclusion chromatography of proteins and biopolymers. Its silica-based, diol-bonded stationary phase offers exceptional stability and reproducibility, ensuring reliable molecular size separation without non-specific binding. This column is ideal for applications such as protein aggregate analysis, molecular weight estimation, and quality control of monoclonal antibodies and other biologics.

Designed for efficient operation with both HPLC and UHPLC systems, it delivers accurate and consistent results even with complex biological samples. The Bio SEC-5 column is compatible with Agilent 1100, 1200, 1260, and 1290 series LC systems, as well as other popular HPLC instruments. It pairs seamlessly with Agilent LC detectors and is also suitable for workflows requiring mass spectrometry detection. For broader analytical needs, it can be used alongside other Agilent Bio SEC columns with varying pore sizes and lengths to optimize resolution and throughput. This product supports demanding research and QC environments in pharmaceutical, biotechnology, and academic laboratories, ensuring high performance and robust reproducibility in biomolecular separations.