Agilent, 5190-2526, Agilent Bio SEC-5, 300Å, 7.8 x 300 mm, 5 µm

Agilent Bio SEC-5, 300Å, 7.8 x 300 mm, 5 µm. A size exclusion column for aggregate analysis of peptides and small proteins. Resolving range 5-1,250 kDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 7.8 mm
LC Platform: Stainless Steel
Length: 300 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 5000 Da
Molecular Weight Upper Limit: 1250000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 300 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The Agilent Bio SEC-5 column offers advanced size exclusion chromatography with a 300 angstrom pore size and a 7.8 x 300 mm dimension filled with 5 micron particles. Designed for high-resolution separation of biomolecules such as monoclonal antibodies, proteins, and protein aggregates, this column ensures reliable molecular weight determination and aggregate analysis. Its robust silica-based chemistry delivers excellent stability, minimizing non-specific adsorption and maximizing sample recovery.

This product is ideal for routine quality control, process monitoring, and detailed research in biotechnology and pharmaceutical workflows. It is particularly well suited for analyzing complex biological samples where detection of aggregates or fragments is critical.

Compatibility with a wide range of liquid chromatography systems, including Agilent InfinityLab and 1100/1200/1260/1290 series, ensures seamless integration into existing laboratory setups. Pairing with Agilent’s range of SEC calibration standards and buffers further enhances data reliability and reproducibility. The column also complements Agilent’s protein characterization kits and detectors, supporting comprehensive biotherapeutics development pipelines.

Reliable performance, high resolution, and robust construction make this size exclusion column a preferred choice for laboratories focused on protein analysis and characterization. It supports both method development and routine analysis with confidence and precision.