Agilent, 5190-2528, Agilent Bio SEC-5, 300Å, 4.6 x 300 mm, 5 µm

Agilent Bio SEC-5, 300Å, 4.6 x 300 mm, 5 µm. A size exclusion column for aggregate analysis of peptides and small proteins. Resolving range 5-1,250 kDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 300 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 5000 Da
Molecular Weight Upper Limit: 1250000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 300 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The Agilent Bio SEC-5 column offers robust and high-resolution size exclusion chromatography for biomolecular analysis. With a 300 Angstrom pore size, 4.6 x 300 mm dimensions, and 5 micron particle size, this column is optimized for the separation and characterization of proteins, peptides, and other biomolecules in complex samples. It delivers consistent molecular weight distribution profiles and is ideal for applications such as monoclonal antibody analysis, aggregate detection, and purity assessments in biopharmaceutical research and quality control.

Engineered for superior sample recovery and minimal nonspecific binding, the column ensures accurate and reproducible results, even with challenging analytes. It is fully compatible with a range of liquid chromatography instruments, including Agilent 1100, 1200, and 1260 Infinity series, as well as other leading HPLC and UHPLC systems. Integration with Agilent detectors and data systems streamlines workflows for researchers performing in-depth protein or peptide studies.

The column works well with compatible SEC mobile phases and can be paired with other Agilent Bio columns for comprehensive biomolecule profiling. Its durable construction and reliable performance make it a dependable choice for laboratories specializing in proteomics, biotechnology, and pharmaceutical development.