Agilent, 5190-2540, Agilent Bio SEC-5, 1000Å, 7.8 x 50 mm, 5 µm, guard

Agilent Bio SEC-5, 1000Å, 7.8 x 50 mm, 5 µm, guard. A size exclusion guard column to protect and extend the lifetime of a longer analytical SEC column.

Specifications:
Brand: Agilent Bio SEC-5
Guard Column: Yes
Hardware: SS
Inner Diameter (ID): 7.8 mm
LC Platform: Stainless Steel
Length: 50 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 50000 Da
Molecular Weight Upper Limit: 7500000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 1000 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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Designed for high-resolution size exclusion chromatography, this Bio SEC-5 guard column features 1000 angstrom pore size, 7.8 x 50 mm dimensions, and 5 micron particle size, providing robust protection and prolonged lifetime for analytical columns. It delivers reliable separation and analysis of biological macromolecules such as proteins, peptides, and nucleic acids, making it ideal for biomolecular characterization, aggregation monitoring, and purity assessment in pharmaceutical, biotechnological, and academic research settings.

Optimized for compatibility with standard high-performance liquid chromatography (HPLC) and ultra-high-performance liquid chromatography (UHPLC) systems, this guard column integrates seamlessly with corresponding analytical columns in the Bio SEC-5 series for consistent and reproducible results. Its advanced bonded silica stationary phase ensures minimal non-specific interactions, leading to accurate molecular weight determinations and efficient sample throughput.

Engineered for compatibility with Agilent’s 1260 Infinity II and 1290 Infinity LC systems, as well as other mainstream chromatographic platforms, it is recommended to pair this guard with Agilent SEC-5 analytical columns to maximize column lifetime and maintain peak performance. Whether used in research laboratories or biopharmaceutical QA/QC environments, this guard column enables precise, reliable protein separations and robust column protection during routine or demanding applications.