Agilent, 5190-6864, Agilent Bio SEC-5, 150Å, 21.2 x 300 mm, 5 µm

Agilent Bio SEC-5, 150Å, 21.2 x 300 mm, 5 µm. A preparative scale size exclusion column for the purification of biological samples. Resolving range 0.5-150 kDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 21.2 mm
LC Platform: Preparative HPLC
Length: 300 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 500 Da
Molecular Weight Upper Limit: 150000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisPeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 150 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115709
pH Range: 2-8.5

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The Agilent Bio SEC-5 column with a 150A pore size and dimensions of 21.2 x 300 mm is engineered for high-resolution size exclusion chromatography of biomolecules, including proteins and antibodies. Utilizing 5 µm particle size technology, this column delivers superior separation and precise molecular weight determination while minimizing sample dilution. It is well suited for preparative scale purification and characterization tasks in biopharmaceutical research, quality control, and protein engineering. The 150A pore size optimally accommodates a wide molecular weight range, ensuring accurate elution of monomers, aggregates, and other size variants. Designed for compatibility with a variety of Agilent HPLC and UHPLC systems, this column is also suitable for use with other major liquid chromatography platforms. Typical applications include aggregate analysis, protein therapeutic purification, and the study of protein-protein interactions. Its robust silica-based packing is chemically stable, offering reliable performance across multiple runs. The column integrates seamlessly with Agilent's comprehensive SEC portfolio, providing researchers with consistent and reproducible results for downstream workflows. Ideal for laboratories seeking efficient biomolecule separation and precision analytics, this column supports advanced research and routine analysis alike.