Agilent, 5190-6875, Agilent Bio WAX, NP3, 4.6 x 150 mm, 3 µm

Agilent Bio WAX, NP3, 4.6 x 150 mm, 3 µm. A weak anion exchange HPLC column with nonporous particles. Particle size is 3 μm. Nonporous particles are best for efficient mass transfer and high resolution separations.

Specifications:
Brand: Agilent Bio WAX
Column Material: Stainless Steel
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 150 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: OligosProteins
Particle Size: 3 µm
Particle Type: Non-porous
Phase: WAX
Pressure Rating: 551 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-12

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The Agilent Bio WAX NP3 column, sized at 4.6 x 150 mm with 3 micron particle size, is designed for high-performance anion exchange chromatography of biomolecules. Featuring a robust, polymer-based stationary phase, this column delivers excellent separation of proteins, peptides, nucleic acids, and other polar biomolecules under mild, non-denaturing conditions. Its optimized pore structure and particle size ensure high resolution and efficient sample throughput, making it ideal for research and quality control applications in the biopharmaceutical, biotechnology, and academic sectors.

This column is particularly well-suited for analyzing charge variants of monoclonal antibodies, the characterization of oligonucleotides, and the purification of other complex biomolecular mixtures. It is compatible with a wide range of mobile phases, including both aqueous buffers and organic modifiers, offering flexibility across diverse application requirements. The column integrates seamlessly with Agilent HPLC and UHPLC systems, including the 1100, 1200, 1260 Infinity, and 1290 Infinity series, as well as other major chromatographic platforms. For extended workflows, it works well in tandem with Agilent’s sample preparation products and detection modules, providing comprehensive solutions for biomolecule analysis. This makes it a reliable choice for rigorous, reproducible analytical separations in demanding laboratory environments.