Agilent, 5190-6876, Agilent Bio WAX, NP5, 10 x 250 mm, 5 µm

Agilent Bio WAX, NP5, 10 x 250 mm, 5 µm. A weak anion exchange semi-prep sized HPLC column with nonporous particles. Particle size is 5 μm. Nonporous particles are best for efficient mass transfer and high resolution separations.

Specifications:
Brand: Agilent Bio WAX
Column Material: Stainless Steel
Hardware: SS
Inner Diameter (ID): 10 mm
LC Platform: Preparative HPLC
Length: 250 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: OligosProteins
Particle Size: 5 µm
Particle Type: Non-porous
Phase: WAX
Pressure Rating: 413 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115709
pH Range: 2-12

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The Agilent Bio WAX NP5 column is engineered for high-resolution anion-exchange chromatography, ideal for the separation of biomolecules such as nucleic acids, oligonucleotides, and proteins. Featuring a 10 x 250 mm dimension and 5 µm particle size, this column delivers robust performance and reproducible results for research and quality control laboratories. Its proprietary weak anion-exchange chemistry offers excellent peak symmetry and high capacity, making it suitable for charge variant analysis, profiling monoclonal antibodies, and characterizing complex biological samples.

Optimized for use with Agilent InfinityLab, 1200 Series, 1260, and 1290 HPLC systems, the column can also be integrated seamlessly with other leading liquid chromatography instruments. It is compatible with a wide range of mobile phases and is fully suited for gradient and isocratic elution methods. Typical applications include therapeutic protein charge heterogeneity assessment, DNA and RNA purification, and biopharmaceutical development workflows.

For enhanced bioseparations, this column can be paired with the Agilent suite of bio columns and consumables. The rugged construction ensures repeatable results over multiple runs, supporting rigorous analytical protocols required in pharmaceutical and biotechnological research.