Agilent, 5190-6877, Agilent Bio WAX, NP5, 21.2 x 250 mm, 5 µm

Agilent Bio WAX, NP5, 21.2 x 250 mm, 5 µm. A weak anion exchange prep sized HPLC column with nonporous particles. Particle size is 5 μm. Nonporous particles are best for efficient mass transfer and high resolution separations.

Specifications:
Brand: Agilent Bio WAX
Column Material: Stainless Steel
Hardware: SS
Inner Diameter (ID): 21.2 mm
LC Platform: Preparative HPLC
Length: 250 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: OligosProteins
Particle Size: 5 µm
Particle Type: Non-porous
Phase: WAX
Pressure Rating: 413 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115709
pH Range: 2-12

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The Agilent Bio WAX, NP5, 21.2 x 250 mm, 5 µm column is designed for high-resolution, ion-exchange chromatography of biomolecules, offering exceptional performance for the separation of proteins, peptides, and nucleic acids. With a 21.2 mm internal diameter and a length of 250 mm, this column provides optimal capacity and peak resolution, making it ideal for demanding analytical and preparative scale applications. The 5 µm particle size ensures high efficiency and reproducibility in method development and routine analysis, supporting workflows in pharmaceutical, biotechnology, and academic research environments.

Engineered with high-quality strong anion exchange resin, this column is suitable for the purification and characterization of monoclonal antibodies, oligonucleotides, and various other charged biomolecules. It is compatible with a wide range of HPLC and UHPLC systems, including Agilent 1200 Infinity Series and 1260 Infinity II LC systems, and can be seamlessly integrated into existing workflows. Typical usage scenarios include charge variant analysis, protein isoform separation, and downstream bioprocess monitoring. For optimal results, pairing with Agilent’s comprehensive portfolio of bio-inert LC accessories and reagents is recommended. This column ensures reliable performance, robust reproducibility, and efficient biomolecule separation for advanced research needs.