Agilent, 5190-6882, Agilent Bio SAX, NP5, 10 x 250 mm, 5 µm

Agilent Bio SAX, NP5, 10 x 250 mm, 5 µm. A strong anion exchange semi-prep sized HPLC column with nonporous particles. Particle size is 5 μm. Nonporous particles are best for efficient mass transfer and high resolution separations.

Specifications:
Brand: Agilent Bio SAX
Column Material: Stainless Steel
Hardware: SS
Inner Diameter (ID): 10 mm
LC Platform: Preparative HPLC
Length: 250 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: OligosProteins
Particle Size: 5 µm
Particle Type: Non-porous
Phase: SAX
Pressure Rating: 413 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115709
pH Range: 2-12

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Engineered for robust and precise anion exchange separations, this strong anion exchange (SAX) column features a 10 x 250 mm dimension and 5 micron particle size, enabling high-resolution analysis of nucleic acids, proteins, and charged biomolecules. Its non-porous silica substrate provides enhanced mass transfer and reproducibility, making it ideal for demanding bioanalytical workflows. This column supports high-throughput environments, delivering rapid separations with excellent peak symmetry and consistent retention times.

Designed for compatibility with Agilent HPLC and UHPLC systems, it integrates seamlessly into existing chromatographic setups, ensuring reliable performance during qualitative and quantitative assays. Typical usage scenarios include biomolecule characterization, quality control, and research-focused investigations that require sensitive detection and accurate identification of anionic analytes. The column is particularly beneficial in biopharmaceutical development, nucleic acid research, and protein purity assessment.

It is optimized for use with a range of mobile phases, accommodating standard buffers and organic modifiers. For enhanced analytical flexibility, it can be combined with other Agilent Bio columns or paired with Agilent detectors and sample preparation products. This makes it a versatile and reliable choice for laboratories seeking high-performance ion exchange chromatography solutions.