Agilent, 5190-6883, Agilent Bio SAX, NP5, 21.2 x 250 mm, 5 µm

Agilent Bio SAX, NP5, 21.2 x 250 mm, 5 µm. A strong anion exchange prep sized HPLC column with nonporous particles. Particle size is 5 μm. Nonporous particles are best for efficient mass transfer and high resolution separations.

Specifications:
Brand: Agilent Bio SAX
Column Material: Stainless Steel
Hardware: SS
Inner Diameter (ID): 21.2 mm
LC Platform: Preparative HPLC
Length: 250 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: OligosProteins
Particle Size: 5 µm
Particle Type: Non-porous
Phase: SAX
Pressure Rating: 413 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115709
pH Range: 2-12

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The Agilent Bio SAX NP5 column is engineered for high-performance separation of biomolecules based on their charge properties using strong anion exchange chromatography principles. Featuring a robust 21.2 x 250 mm dimension and 5 micrometer particle size, this preparative column offers excellent resolution and capacity, ideal for large-scale purification workflows. It is particularly suited for the fractionation and analysis of proteins, peptides, nucleic acids, and other charged biomolecules in biopharmaceutical, proteomics, and biotechnology laboratories.

Utilizing non-porous resin technology, the column ensures rapid mass transfer and high recovery, enabling efficient processing with minimal sample degradation. Its strong anion exchange functionality provides reproducible separations, even with complex samples, supporting both method development and routine quality control. The design supports high flow rates and pressure tolerance compatible with most preparative high-performance liquid chromatography and FPLC systems.

Frequent applications include process scale purification, aggregate removal, and charge variant analysis, supporting regulatory compliance in therapeutic protein development. This column is fully compatible with Agilent HPLC instruments and commonly used buffer systems. It also integrates seamlessly with other Agilent Bio columns, enabling flexible workflow optimization for diverse analytical and preparative needs in advanced research and production environments.