Agilent, 5190-6885, Agilent Bio MAb, NP5, 21.2 x 250 mm, 5 µm

Agilent Bio MAb, NP5, 21.2 x 250 mm, 5 µm. This prep HPLC column has a densely packed weak cation exchange layer designed for monoclonal antibody separations. The particles are nonporous and promote higher resolution and faster separations.

Specifications:
Brand: Agilent Bio MAb
Column Material: Stainless Steel
Hardware: SS
Inner Diameter (ID): 21.2 mm
LC Platform: Preparative HPLC
Length: 250 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: Charge Variant AnalysisPeptidesProteinsmAbs
Particle Size: 5 µm
Particle Type: Non-porous
Phase: WCX
Pressure Rating: 413 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115709
pH Range: 2-12

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The Agilent Bio MAb NP5 column, measuring 21.2 x 250 mm with a 5 micron particle size, is designed for high-performance purification and analysis of monoclonal antibodies and related biomolecules. Utilizing proprietary NP5 technology, the column delivers excellent resolution and reproducibility in preparative and process-scale chromatography workflows. This solution supports downstream bioprocessing in pharmaceutical development and quality control laboratories, making it ideal for antibody purification, aggregate analysis, and characterization studies. The robust silica-based stationary phase ensures reliable performance even under demanding operating conditions and gradients. Compatible with a range of Agilent high-performance liquid chromatography systems, including preparative HPLC platforms, it integrates seamlessly for scalable workflows from R and D through pilot-scale production. The column also supports compatibility with other major HPLC and FPLC instruments. Its optimized particle and pore structure promotes rapid mass transfer and high recovery yields, facilitating efficient separation of complex protein samples in biotherapeutic research and process monitoring. This prep-scale column can be paired with Agilent’s range of biomolecule buffers and sample preparation accessories to enhance workflow efficiency and robustness across biopharmaceutical applications.