Agilent, 877974-901, ZORBAX GF-250, 21.2 x 250 mm, 6 µm, cartridge (PrepHT)

ZORBAX GF-250, 21.2 x 250 mm, 6 µm, cartridge (PrepHT). Use with hardware kit 820400-901

Specifications:
Brand: ZORBAX
Hardware: SS
Inner Diameter (ID): 21.2 mm
LC Platform: Preparative HPLC
Length: 250 mm
Maximum Temperature: 60 °C
Molecular Weight Lower Limit: 4000 Da
Molecular Weight Upper Limit: 400000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisPeptidesProteins
Particle Size: 6 µm
Particle Type: Fully Porous
Phase: GF-250
Pore Size: 150 Å
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 3-8.5

**********The following product description is generated by AI and is for reference only. If you have any questions, please contact customer service.**********
The ZORBAX GF-250, 21.2 x 250 mm, 6 µm cartridge is designed for preparative high throughput size exclusion chromatography (SEC) applications. Offering efficient separation of biomolecules based on molecular size, it is particularly suitable for the analysis and purification of proteins, peptides, nucleic acids, and other macromolecules in research and pharmaceutical environments. The 6 µm particle size ensures high resolution and reliable performance, making it ideal for both method development and routine purification tasks.

This cartridge format allows for straightforward integration with compatible Agilent Preparative LC systems, including PrepHT hardware, facilitating quick scale-up from analytical to preparative workflows. Its robust design and high-loading capacity make it an excellent choice for laboratories requiring reproducible results with demanding sample loads. The GF-250 media offers optimal pore size for separation of molecules in the 13 to 600 kilodalton (kDa) range, supporting diverse research and bioprocessing needs.

Typical usage scenarios include aggregate analysis, buffer exchange, desalting, and sample concentration prior to downstream analysis. Compatible with a wide range of aqueous mobile phases, this cartridge supports efficient protein purification and quality control. It is part of Agilent's broader ZORBAX line, ensuring seamless method transfer and flexible scaling options.