Agilent, GPPNG-APTS, GlykoPrep-plus N-glycan Prep with APTS, 96-ct

GlykoPrep-plus N-glycan Prep with APTS, 96-ct. Complete kit configured for automation on the Agilent AssayMAP Bravo liquid handling platform. GlykoPrep N-glycan sample preparation is a cartridge based workflow. Includes GSP-RX digestion module, GS96-APTS Reductive Amination APTS labeling module, GS96-C2 cleanup module, AM96-NG GlykoPrep-plus labware set

Specifications:
Count: 96
Equipment & Reagents Provided by User: 100% Acetonitrile (~250 mL, HPLC-grade)Calibrated pipettes and disposable tips (P5/10, P200 and P1000)Fume hoodGlass graduated cylinder (50 mL, 1 ea)Microplate compatible, centrifugal evaporator (e.g., SpeedVac with plate rotor or similar)Nitrile glovesUltrapure, deionized water (~100 mL, Milli-Q or equivalent)Volumetric pipettes (5 mL, 1 ea)Vortexer
Instrument Compatibility: AssayMAP Bravo Platform
Kit Configuration: Sample Prep Kit
Label: APTS
Prep time (approx): 3 hours
Technique: CE

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This kit provides an efficient solution for the preparation and fluorescent labeling of N-linked glycans from glycoprotein samples using 8-aminopyrene-1,3,6-trisulfonic acid (APTS). Designed for high-throughput workflows, each package includes 96 ready-to-use units, supporting rapid parallel processing for biomarker discovery, glycoprotein characterization, and quality control in biopharmaceutical development. The workflow enables robust enzymatic release of N-glycans followed by APTS labeling, facilitating sensitive detection and quantification by capillary electrophoresis with laser-induced fluorescence (CE-LIF) or other fluorescence-based separation techniques. This kit integrates seamlessly with Agilent’s glycan analysis platforms such as 7100 Capillary Electrophoresis System and is compatible with automated liquid handling systems. Applications span research in cell line development, therapeutic antibody profiling, and monitoring of glycosylation patterns for product consistency. The included protocols and reagents are optimized to minimize sample loss and support reproducible results, making it an ideal choice for laboratories needing streamlined N-glycan preparation and analysis. Related consumables and analytical columns for glycan separation are available from Agilent to further enhance your workflow efficiency and data quality.