Agilent, GX24-2AB, AdvanceBio Gly-X N-glycan prep with 2-AB Express, 24-ct

AdvanceBio Gly-X N-glycan prep with 2-AB Express, 24-ct. Complete kit utilizes a novel in-solution enzymatic protein deglycosylation followed by on-matrix Express on-matrix labeling of released N-glycans with 2-AB dye, with no prior drydown. With deglycosylation carried out in solution, the method is simple, rapid, and suitable for automation. After a simple cleanup step, the 2-AB-labeled glycan samples are ready for analysis by UHPLC, LC/MS, MS/MS and other methods. Includes GX24-100 Gly-X deglycosylation module, GX24-401 Gly-X 2-AB Express labeling module, GX96-402 Gly-X 2-AB Express cleanup module (96-ct).

Specifications:
Application: Flexible, high-throughput format:process 1 to 24 samples
Count: 24
Deglycosylation Efficiency: > 99 %
Equipment & Reagents Provided by User: 96-well Thermocycler or two independent heat blocksAcetonitrile (ACN), MS-grade (Fisher A955-4 recommended)DI waterTHERM-1000, 230V)Thermocycler (Corning THERM-1001, 110VVacuum manifold (Millipore MSVMHTS00-HTS)Vacuum manifold spacer (GX100, or GX400 Gly-X 2-AB Starter Kit - included free of charge with first Gly-X order, please contact us for details)Vacuum pump (Millipore WP6211560, 110 VWP6122050, 220VWelch WOB-L Pump 2522)
Kit Configuration: Sample Prep Kit
Label: 2-AB
Precision (well-to-well, day-to-day): <5% CV
Prep time (approx): ~2.5 hours
Preservation of Sialylated Species: > 99 %
Protein Load Range: 1-40 µg
Recovery: > 80 %
Stability: At least 1 month at -20°C, 5 days at 4–8°C
Technique: LC & LC/MS

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The AdvanceBio Gly-X N-glycan prep with 2-AB Express, 24-count kit provides a streamlined solution for reliable and reproducible enzymatic deglycosylation and labeling of N-glycans using 2-aminobenzamide (2-AB) fluorescence. Ideal for high-throughput laboratories, this kit enables rapid sample preparation for quantitative glycan profiling in biopharmaceutical quality control and glycomics research. The included reagents support fast and consistent N-glycan release, followed by efficient labeling and cleanup, minimizing workflow variability and hands-on time. This kit is fully compatible with Agilent HPLC, UHPLC, and LC-MS systems, ensuring optimal performance with AdvanceBio Glycan columns. It is also suitable for automation on liquid handling platforms, making it adaptable for both manual and high-throughput workflows. Researchers can confidently use these prepared samples for applications such as glycoprotein characterization, biosimilar comparability, lot-to-lot consistency studies, and biomarker discovery. The kit’s optimized protocol reduces preparation to less than one hour and offers sensitive detection for low-abundance glycan species. For best results, it may be used alongside complementary AdvanceBio sample preparation, standards, and analytical solutions designed to support comprehensive glycan analysis from sample to result.