Agilent, GX24-IPC, AdvanceBio Gly-X N-glycan prep with InstantPC Kit, 24-ct

AdvanceBio Gly-X N-glycan prep with InstantPC Kit, 24-ct. Complete kit utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantPC dye. After a simple cleanup step, the glycan samples are ready for analysis by UHPLC, LC/MS, MS/MS, and other methods. With deglycosylation and labeling carried out in solution, the method is simple, rapid, and suitable for automation. The InstantPC dye delivers unmatched fluorescent brightness and MS signal, which enables a single labeling method to be deployed across different glycan analysis workflows. Sample preparation time: ~1 hour. Includes GX24-100 Gly-X deglycosylation module, GX24-101 Gly-X InstantPC labeling module, GX96-102 Gly-X InstantPC cleanup module.

Specifications:
Application: Flexible, high-throughput format:process 1 to 24 samplesNote:The 24-count kit includes a 96-count cleanup plate. This plate can be stored at room temperature for future use. Gly-X InstantPC digestion and labeling reagents (GX24-201PC) are available separately for use with existing cleanup plates.Other benefits include:
Count: 24
Equipment & Reagents Provided by User: 96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C. (e.g. Corning THERM-1001, 110V. THERM-1000, 230V). Note:Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocksVacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522). Note:If you have a vacuum manifold and pump other than the Millipore model suggested, please contact us for information. Alternative vacuum manifold may be used with a GX200 spacer in place of GX100 (see FAQs)
Kit Configuration: Sample Prep Kit
Label: InstantPC
Precision (well-to-well, day-to-day): <5% CV
Prep time (approx): 42 min (16 samples), 99 min (96 samples)
Preservation of Sialylated Species: > 95 %
Protein Load Range: 1-40 µg
Recovery: > 95 %
Stability: Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF
Technique: LC & LC/MS

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The AdvanceBio Gly-X N-glycan prep with InstantPC Kit streamlines the preparation and fluorescent labeling of N-linked glycans for high-throughput analysis in biopharmaceutical research and quality control environments. Designed for consistent performance, this kit enables rapid enzymatic release and efficient labeling of glycans using the InstantPC chemistry, ensuring high sensitivity and reproducibility in downstream analyses. The easy-to-use workflow significantly reduces sample preparation time while minimizing sample loss, making it ideal for glycan profiling, characterization, and comparability studies of therapeutic monoclonal antibodies and other glycoproteins.

The kit supports up to 24 samples and is optimized for seamless integration with liquid chromatography and mass spectrometry platforms, including Agilent HPLC, UHPLC, and LC/MS systems. Researchers benefit from enhanced fluorescent signal intensity, enabling accurate quantitation and robust detection of low-abundance glycan species. The included InstantPC reagent provides superior labeling efficiency compared to conventional dyes, facilitating confident identification and structural elucidation of glycans during method development or routine monitoring.

This workflow is compatible with Agilent AdvanceBio columns and related glycan analysis solutions, supporting regulatory compliance and accelerating development timelines. The kit is recommended for laboratories conducting glycosylation analysis in biologics research, bioprocess development, and lot release testing.