BD, 550295, BD Pharmingen™ Purified Mouse Anti-Rat CD3

Alternative Name: CD3 Complex; T3
Reactivity: Rat (QC Testing)
Isotype: Mouse BALB/c IgG3, κ
Immunogen: PHA-stimulated rat lymph node and spleen cells
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development), Cytotoxicity, Immunoprecipitation, Inhibition, Stimulation (Reported)
Concentration: 31.25 µg/ml
RRID: AB_393590
Storage Buffer: Aqueous buffered solution containing BSA, protein stabilizer, goat serum, and ≤ 0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: Purified Mouse Anti-Rat CD3 (Cat. No. 550295) is recommended to test for immunohistochemical staining of acetone-fixed frozen sections and paraffin sections. Tissue tested was rat spleen. The antibody stains T lymphocytes. The isotype control recommended for use with this antibody is Purified Mouse IgG3, κ Isotype Control (Cat. No. 550341). For optimal indirect immunohistochemical staining, the G4.18 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with Purified Mouse IgG3, κ Isotype Control (Cat. No. 553401) as the secondary antibody and Streptavidin HRP (Cat. No. 550946) together with the DAB Substrate Kit (Cat. No. 550880).