BD, 550333, BD Pharmingen™ Biotin Rat Anti-Mouse IgG2b

Alternative Name: Igh-3; gamma2b; Immunoglobulin heavy constant gamma 2B
Reactivity: Mouse (QC Testing)
Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
Immunogen: Pooled Mouse Ig
Application: ELISA (Routinely Tested), Flow cytometry, Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development)
Concentration: 250 µg/ml
RRID: AB_393613
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Recommended Assay Procedures: Recommended Assay Procedures This antibody conjugate has been tested as a detecting reagent in ELISA (~2 µg/ml) to assure purity and reactivity. For immunohistochemical staining, a dilution range of 1:25 - 1:100 is suggested on acetone-fixed frozen sections.