BD, 550350, BD Pharmingen™ Purified Rat Anti-Mouse Syndecan-4

Reactivity: Mouse (QC Testing)
Isotype: Rat WI, also known as Wistar (outbred) IgG2a, κ
Immunogen: BCB10 mouse pre-B cell line
Application: Flow cytometry (Routinely Tested), ELISA (Reported)
Concentration: 0.5 mg/ml
RRID: AB_393629
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures We recommend the use of Mouse Fc Block™ (anti-mouse CD16/CD32, mAb 2.4G2, Cat. No. 553141/553142) to reduce non-specific staining. For staining in the presence of Mouse Fc Block™, the second-step antibody must not recognize the 2.4G2 antibody (rat IgG2b, κ). Therefore, we recommend biotinylated mouse anti-rat IgG2a (Cat. no. 553894) as a secondary antibody, followed by Streptavidin-PE (Cat. No. 554061).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.