BD, 550374, BD Pharmingen™ Purified Mouse Anti-Human CD11b

Alternative Name: MAC-1A; Mac-1; ITGAM; Integrin alpha M; CR3A; CR-3 alpha; Mo1; SLEB6
Reactivity: Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
Isotype: Mouse IgG1, κ
Immunogen: Human monocytes
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Western blot (Reported), Immunohistochemistry-paraffin (Not Recommended)
Concentration: 62.5 µg/ml
Workshop Number: IV M047
RRID: AB_393645
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The ICRF44 (44) antibody is recommended to test for immunohistochemical staining of acetone-fixed frozen sections. Tissue tested was human spleen and tonsil. The antibody stains activated lymphocytes, monocytes, granulocytes and a subset of NK cells. The isotype control recommended for use with this antibody is Purified Mouse IgG1 κ Isotype Control (Cat. No. 550878). For optimal indirect immunohistochemical staining, the ICRF44 (44) antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with Biotin Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550337) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). The clone ICRF44 (44) is not recommended for formalin-fixed paraffin embedded sections.