BD, 550428, BD Pharmingen™ Purified Mouse Anti-Human IRF-3

Alternative Name: Interferon regulatory factor 3; IRF-3; IRF3; IIAE7
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1, κ
Immunogen: Human IRF-3 aa. 56-427 Recombinant Fusion Protein
Application: Western blot (Routinely Tested), Blocking, Immunofluorescence, Immunoprecipitation (Reported)
Target Molecular Weight: 50 kDa
Concentration: 0.5 mg/ml
RRID: AB_393677
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Clone SL-12.1 can be used for western blot analysis (0.5-2.0 µg/ml). Other reported applications include immunoprecipitation and immunofluorescence microscopy. The antibody will recognize both the nuclear and cytoplasmic forms of IRF-3 and can block IRF-3 DNA binding, although this application has not been tested at BD Biosciences. Jurkat cells are recommended as a positive control (Cat. No. 611451).