BD, 550486, BD Pharmingen™ Purified Mouse Anti-Human MUC1 (CD227)

Alternative Name: MUC1; CA 15-3; DF3; EMA; Episialin; H23; H23AG; KL-6; MAM6; PEM; PEMT; PUM
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1, κ
Immunogen: Human Milk Fat Globule Membranes
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen (Tested During Development)
Concentration: 500 µg/ml
RRID: AB_2148558
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The HMPV clone reactive against human MUC1 is recommended to test for immunohistochemical staining of formalin-fixed paraffin and acetone-fixed frozen sections. For paraffin sections, microwave pretreatment with BD Retrievagen A (pH 6.5) (Cat. No. 550524) is required. Tissue tested was human breast cancer tissue. The antibody stains normal and cancerous cells with a high carbohydrate content. The isotype control recommended for use with this antibody is purified mouse IgG1 (Cat. No. 550878). For optimal indirect immunohistochemical staining, HMPV antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-mouse Igs (multiple adsorbed) (Cat. No. 550337) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). More conveniently, the Anti-Mouse Ig HRP detection kit (Cat. No. 551011) that contains the biotinylated secondary antibody, antibody diluent, Streptavidin-HRP and DAB substrate can be used for staining. A detailed protocol of the immunohistochemical procedure is available at our website, http://www.bdbiosciences.com/support/resources.