BD, 550541, BD Pharmingen™ Purified Hamster Anti-Mouse CD61

Alternative Name: Itgb3; Integrin beta-3; INGRB3; Platelet glycoprotein IIIa; GP3A; GPIIIa
Reactivity: Mouse (QC Testing), Rat (Tested in Development)
Isotype: Armenian Hamster IgG1, κ
Immunogen: Mouse T-cell Hybridoma 2B4 Vitronectin Receptor
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-paraffin (Not Recommended)
Concentration: 250 µg/ml
RRID: AB_393735
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The 2C9.G2 antibody specific for mouse CD61 is recommended to test for immunohistochemical staining of acetone-fixed frozen sections. Tissues tested were mouse spleen and thymus. The antibody stains platelets activated T lymphocytes and polymorphonuclear granulocytes. The isotype control recommended for use with this antibody is purified hamster IgG (Cat. No. 550344). For optimal indirect immunohistochemical staining, the 2C9.G2 antibody should be titrated (1-10 to 1-50 dilution and visualized via a three-step staining procedure in combination with anti-hamster IgG cocktail (Cat. No. 550335) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). A detailed protocol of the immunohistochemical procedure is available at our website, http://www.bdbiosciences.com/support/resources
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf. An isotype control should be used at the same concentration as the antibody of interest. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.