BD, 551121, BD Pharmingen™ Purified Mouse Anti-Human CD194

Alternative Name: CCR4; C-C chemokine receptor type 4; CMKBR4; K5-5
Reactivity: Human (QC Testing)
Isotype: Mouse C57BL/6 IgG1, κ
Immunogen: Human CCR4 Transfected Cell Line
Application: Flow cytometry (Routinely Tested)
Concentration: 0.5 mg/ml
Workshop Number: HCDM 2006
RRID: AB_2074502
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Flow cytometry: Chemokine receptors are known to internalize during manipulation resulting in low frequency expression.  Investigators are advised to perform immunophenotyping studies of chemokine receptors on freshly collected samples (<24 Hrs). Incubation with the antibody should be done at 4°C in the dark. Cellular manipulation, such as Ficoll separation, freezing, or exposure to cold temperatures prior to staining should be minimized and have been shown to cause a decrease in staining intensity and/or inconsistent results. Investigators should note that a multiple-step staining procedure is recommended, in some instances, to amplify immunofluorescent signals for the flow cytometric analysis of CCR4 expression.  Investigators may find the Purified Mouse Anti-Human CD194 (CCR4) antibody (MN 551121) to be useful in conjunction with appropriate secondary and tertiary reagents for detecting low frequency expression, such as with Biotin Goat Anti-Mouse Ig (MN 553999) and PE Streptavidin (MN 554061).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.