BD, 551964, BD Pharmingen™ Purified Mouse Anti-Human Toll-Like Receptor 4

Alternative Name: TLR4; CD284; Toll-like Receptor 4; ARMD10
Reactivity: Human (QC Testing)
Isotype: Mouse BALB/c IgG2a, κ
Immunogen: Human TLR4-transfected Ba/F3 cell line
Application: Flow cytometry (Routinely Tested), Blocking (Reported)
Concentration: 0.5 mg/ml
RRID: AB_394304
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures A multistep step staining procedure is recommended to amplify immunofluorescent signals for the flow cytometric analysis of human TLR4 expression: Step 1: Incubate 10^6 cells with 0.25 - 1.0 µg/test of purified HTA125 antibody at 4°C for at least 15 - 20 minutes. Wash cells two times with staining medium containing sodium azide (e.g., BD Pharmingen™ Stain Buffer, Cat. No. 554656). Step 2: Incubate the cells with biotinylated anti-mouse Ig  (Cat. No. 553999) or biotinylated anti-mouse IgG2a, (Cat. No. 553388) at 4°C for 20 minutes. Wash cells two times. Step 3: Incubate the cells with  ≤0.06 µg of streptavidin-phycoerythrin (Cat. No. 554061) at 4°C for 20 minutes. Wash two times. Resuspend cells in stain buffer and analyze stained cells by flow cytometry using appropriate specificity and compensation controls.