BD, 552176, BD Pharmingen™ PE Rat Anti-Human CCR7 (CD197)

Alternative Name: CCR7, BLR-2, EBI-1, CMKBR7
Reactivity: Human (QC Testing)
Isotype: Rat IgG2a, κ
Immunogen: Human CCR7 protein
Application: Flow cytometry (Routinely Tested)
Vol. Per Test: 20 µl
RRID: AB_394354
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures The PE-conjugated 3D12 antibody is convenient for the immunofluorescent staining and flow cytometric analyses of human leukocytes and cell lines that express CCR7 (see figure). Chemokine receptors are known to internalize during manipulation resulting in low frequency expression. Immunophenotyping studies of chemokine receptors need to be performed on freshly collected whole blood (<24 hours). Incubation with the antibody should be done at 4°C in the dark. Cellular manipulation, such as Ficoll separation, freezing, or exposure to cold temperatures prior to staining have been shown to cause a decrease in staining intensity and inconsistent results. A multiple-step staining procedure is strongly recommended to amplify immunofluorescent signals in the flow cytometric analysis of human CCR7 expression. Purified Rat Anti-Human CCR7 (CD197) (Cat. No. 552175) and a 3-step staining procedure is recommended for detecting low frequency expression.