BD, 552560, BD Pharmingen™ Biotin Mouse Anti-Human CD154

Alternative Name: CD40L
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1, κ
Application: ELISA Detection (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_394429
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Recommended Assay Procedures: Recommended Assay Procedures ELISA Capture: Biotin Mouse Anti-Human CD154 antibody (Cat. No. 552560) is useful as a detection antibody for a sandwich ELISA for measuring human CD154 (CD40L) protein levels.Biotin Mouse Anti-Human CD154 antibody should be titrated between 0.5 - 2 µg/ml to determine its optimal concentration for ELISA detection. To obtain linear standard curves, doubling dilutions of recombinant human soluble CD154 (CD40L), ranging from 2000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology see the protocols under "ELISA and ELISPOT" at our website: http://www.bdbiosciences.com/en-us/support/bd-biosciences-resource-library. Note: This ELISA antibody pair shows no cross-reactivity with the following recombinant human cytokines: CD40, IL-1Ra, IL-1R1, IL-1RII, IL-1α, IL-1β, IL-2, IL-3, IL-4, sIL-4R, IL-5, IL-6, IL-6R, IL-7, IL-8, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, TNF-α, TNF-β, sTNFRI, sTNFRII, IFNγ, TRAIL, GM-CSF, TGFβ, Trx