BD, 553000, BD Pharmingen™ Purified Rat Anti-Mouse CD90.2

Alternative Name: Thy-1.2; T25; Thymus cell antigen 1, theta
Reactivity: Mouse (QC Testing)
Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
Immunogen: Mouse Thymus / Spleen
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Cytotoxicity, Electron microscopy, Immunoprecipitation (Reported)
Concentration: 0.5 mg/ml
RRID: AB_394539
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Flow Cytometry: Mouse BD Fc Block™ purified anti-mouse CD16/CD32 clone 2.4G2 (Cat. No. 553141) may help to reduce non-specific binding to cells bearing Fcγ-receptors.  For staining in the presence of Mouse BD Fc Block™, a second step antibody which does not recognize the 2.4G2 mAb (Rat IgG2b, κ) must be used, such as FITC anti-rat IgG2a, mAb RG7/1.30 (Cat. No. 553896). Caution: Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also effect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format for in vitro and in vivo use.