BD, 554155, BD Pharmingen™ Purified Mouse Anti-Adenovirus Type 5 E1A

Reactivity: Human (QC Testing)
Isotype: Mouse IgG2a
Immunogen: A trpE-E1A fusion protein
Application: Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Reported)
Concentration: 0.5 mg/ml
RRID: AB_395274
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Applications include western blot analysis (1-2 µg/ml), immunoprecipitation (1-2 µg/one million cells) 4and indirect immunofluorescence of cultured cells (5-20 µg/ml). The E1A proteins migrate at various molecular weights depending on mRNA processing and posttranslational modifications; at least 60 E1A polypeptide species have been identified, ranging between 30 and 46 kDa. Two species resulting from the 12S and 13S E1A mRNAs have reduced molecular weights of 26 kDa and 32 kDa, respectively. 293 human embryonic kidney cells or E1A/Adenovirus Type 5 infected HeLa cells are suggested as positive controls.