BD, 554164, BD Pharmingen™ Purified Mouse Anti-Human Underphosphorylated Retinoblastoma Protein (RB)

Alternative Name: RB (a.a. 514-610)
Reactivity: Human (QC Testing), Mouse (Tested in Development)
Isotype: Mouse IgG1
Immunogen: Recombinant full-length human Rb
Application: Immunoprecipitation (Routinely Tested)
Target Molecular Weight: 105 kDa
Concentration: 0.5 mg/ml
RRID: AB_395279
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Applications include immunoprecipitation at 1-2 µg/one million cells. Rb migrates as multiple, closely-spaced bands between approximately 110-116 kDa when sized on denaturing polyacrylamide gels (i.e. by SDS/PAGE). The multiple bands represent different Rb phosphorylation states, the higher molecular weight bands are more highly phosphorylated than the lower molecular weight bands. The level of phosphorylation is cell cycle dependent, and may also be cell type dependent (not all forms are seen in all cell types that express Rb). Gel conditions influence the actual number of bands observed. In cases where optimal band separation is desired, use a 7 to 10% non-gradient long (at least 12 inches) gel. MOLT-4 human leukemia cells (ATCC CRL-1582) are suggested as a positive control.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.