BD, 554223, BD Pharmingen™ Purified Mouse Anti-Human DCC

Reactivity: Human (QC Testing)
Isotype: Mouse IgG1
Immunogen: Recombinant Human DCC
Application: Western blot (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required) (Reported)
Target Molecular Weight: 175-190 kDa
Concentration: 0.5 mg/ml
RRID: AB_395314
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Applications include western blot analysis (0.5 - 2.0 µg/ml). IMR-32 (ATCC CCL-127) cells are suggested as a positive control. The antibody has also been published for immunohistochemistry of formalin-fixed, paraffin-embedded tissue sections.  By western blot, DCC-specific antibodies typically identify protein species with molecular weights of approximately 175-190 kDa. Doublets in this range have been reported in brain.  Several smaller immunoreactive species, representing degradation products, cross-reactive species, or DCC forms arising from alternative splicing of DCC mRNA or in vivo processing of the DCC protein may also be identified.