BD, 554336, BD Pharmingen™ Recombinant Soluble Human Fas (FasΔTM)

Preparation And Storage: Preparation And Storage Avoid multiple freeze-thaws of product. Store the protein at -20°C, or for longer storage life, at -80°C.
Recommended Assay Procedures: Recommended Assay Procedures Purified FasΔTM can be used as a positive control for western blot analysis of Fas. 1 µg of protein can easily be visualized on a Commasie-stained gel as a band of ~40 kDa. For western blot analysis, 1 µg or less is sufficient depending on the detection method (less is needed for enhanced chemiluminescence than for standard alkaline phosphatase detection). Purified FasΔTM can also be used as a standard in Fas ELISA assays. The recombinant FasΔTM protein should not be used in functional assays, as it does not appear to assume a biologically active conformation.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Licensed for Research Purposes Only. Commercial use requires license from Boyce Thompson Institute for Plant Research. Source of all serum proteins is from USDA inspected abattoirs located in the United States.