BD, 555055, BD Pharmingen™ Purified Mouse Anti-Human MCP-1

Reactivity: Human (QC Testing)
Isotype: Mouse IgG1, κ
Immunogen: E-coli-expressed recombinant human MCP-1
Application: ELISA Capture (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_395676
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures ELISA Capture : The purified 10F7 antibody is useful as a capture antibody for a sandwich ELISA for measuring human MCP-1 protein levels. The purified 10F7 antibody can be paired with the biotinylated anti-human MCP-1 monoclonal antibody 5D3-F7 (Cat. No. 554664) as the detection antibody with recombinant human MCP-1 (Cat. No. 554620) as the standard. Purified 10F7 antibody should be titrated 2-6 µg/ml to determine the optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of human MCP-1 ranging from ~5000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit the chapter on ELISA in the Immune Function Handbook, which is posted on our website at www.bdbiosciences.com. Note 1: This ELISA pair shows no cross-reactivity with any of the following chemokines tested (e.g., Human eotaxin, MCP-2, MCP-3, MCP-4, mouse MCP-1 and rat MCP-1). Note 2: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum samples. BD OptEIA™ ELISA Set Cat. No. 555179 and Kit 559017 are specially-formulated for serum cytokine measurement.