BD, 555896, BD Pharmingen™ Purified Rat Anti-Human CD132

Alternative Name: IL-2RG; IL-2Rγ; γc; Common γ chain; Common gamma chain; gamma c; SCIDX1
Reactivity: Human (QC Testing), Dog (Tested in Development)
Isotype: Rat IgG2b, κ
Immunogen: Human γc Transfected Cell Line
Application: Flow cytometry (Routinely Tested)
Concentration: 0.5 mg/ml
Workshop Number: VI C-89
RRID: AB_396208
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures This reagent is effective for indirect immunofluorescence staining of human tissue for flow cytometric analysis. For flow cytometric applications, a three step labelling procedure is recommended for amplifying signal. Suggested protocol for 3-step staining using Lysed Whole Blood method: 1. Incubate 100 µl whole blood with primary (unconjugated) antibody for 20-30 minutes at room temperature. 2. Add 2 mls of 1X Pharm Lyse (10X Pharm Lyse, Cat. No. 555899) and incubate for 10-15 minutes. Centrifuge and aspirate. 3. Wash once with PBS/0.1% sodium azide/ 1% heat-inactivated fetal bovine serum (PBS-FBS). Centrifuge and aspirate. 4. Add biotinylated mouse anti-rat Ig's (Cat. No. 553883) and incubate for 20-30 minutes at room temperature. 5. Wash once with PBS-FBS. Centrifuge and aspirate. 6. Add SAV-PE (Cat. No. 554061) and incubate for 20-30 minutes in the dark at room temperature. 7. Wash once with PBS-FBS. Centrifuge and aspirate Resuspend in 0.5 ml of PBS-FBS and analyze by flow cytometry.