BD, 557539, BD Pharmingen™ Purified NA/LE Hamster Anti-Mouse IL-1β

Reactivity: Mouse (QC Testing)
Isotype: Armenian Hamster IgG, λ1
Immunogen: Mouse IL-1β Protein
Application: ELISA (Routinely Tested), Neutralization (Tested During Development), Immunoprecipitation (Reported)
Concentration: 1.0 mg/ml
RRID: AB_396752
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Recommended Assay Procedures: Recommended Assay Procedures ELISA : Purified Hamster Anti-Mouse IL-1β (Cat. No. 550605) has been reported to be useful as a capture antibody for sandwich ELISA measuring mouse IL-1 β protein levels.   For measuring mouse IL-1β in complex biological samples, such as serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Mouse IL-1β ELISA Set (Cat. No 559603). Immunoprecipitation :  Purified Hamster Anti-Mouse IL-1β (clone B122) has been reported to be useful for the immunoprecipitation of both natural and recombinant forms of mouse IL-1 β protein.  Please note that this antibody is not routinely tested for this application.